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An Overview of the Loading Efficacy of Cationic Lipids with Milk Proteins: A Potential Application for Lipid Delivery

Author(s):

P. Chanphai, Philippe Bourassa and Heidar -Ali Tajmir-Riahi*   Pages 1 - 9 ( 9 )

Abstract:


In this review, the loading efficacies of helper and cationic lipids cholesterol (CHOL), 1,2-dioleoyl-3-trimethylammonium-propane (DOTAP), dioctadecyl dimethylammonium bromide (DDAB) and dioleoyl phosphatidylethanolamine (DOPE) with milk β- lactoglobulin, α-casein and β-casein were compared in aqueous solution at physiological conditions. Structural analysis showed that lipids bind milk proteins via hydrophilic, hydrophobic and H-bonding contacts with DOTAP and DDAB forming more stable protein conjugates. Loading efficacy was 30-50% and enhanced with cationic lipids. Lipid conjugation altered protein conformation, causing a partial protein structural destabilization. Milk proteins are capable of transporting lipids in vitro.

Keywords:

milk protein, lipid, loading efficacy, spectroscopy, modeling

Affiliation:

University of Quebec a Trois-Rivieres, - Department de Chemistry-Biochemistry, Trois-Rivieres, University of Quebec a Trois-Rivieres, - Department de Chemistry-Biochemistry, Trois-Rivieres, University of Quebec a Trois-Rivieres, - Department de Chemistry-Biochemistry, Trois-Rivieres



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