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Protein Microarrays: Valuable Tools for Ocular Diseases Research

Author(s):

María Garranzo-Asensio, Ana Montero-Calle, Guillermo Solís-Fernández, Rodrigo Barderas and Ana Guzman-Aranguez*   Pages 1 - 17 ( 17 )

Abstract:


The eye is a complex organ constituted by several compartments with exclusive and specialized properties that reflect their diverse functions. Although the prevalence of eye pathologies is increasing, mainly because of its correlation with aging and of generalized lifestyle changes, the pathogenic molecular mechanisms of many common ocular diseases remain poorly understood. Therefore, there is an unmet need to delve into the pathogenesis, diagnosis, and treatment of eye diseases to preserve ocular health and reduce the incidence of visual impairment or blindness. Proteomics analysis stands as a valuable tool for deciphering protein profiles related to specific ocular conditions. In turn, such profiles can lead to real breakthroughs in the fields of ocular science and ophthalmology. Among proteomics techniques, protein microarray technology stands out by providing expanded information using very small volumes of samples.

In this review, we present a brief summary of the main types of protein microarrays and their application for the identification of protein changes in chronic ocular diseases such as dry eye, glaucoma, age-related macular degeneration or diabetic retinopathy. The validation of these specific protein alterations could provide new biomarkers, disclose eye diseases pathways and help in the diagnosis and the development of novel therapies for eye pathologies.

Keywords:

protein microarrays, ocular pathology, dry eye, glaucoma, age-related macular degeneration, diabetic retinopathy

Affiliation:

Department of Biochemistry and Molecular Biology, Faculty of Optics and Optometry, Universidad Complutense de Madrid, Madrid, Functional Proteomics Unit, Chronic Disease Programme (UFIEC), Instituto de Salud Carlos III, Majadahonda, Madrid, Functional Proteomics Unit, Chronic Disease Programme (UFIEC), Instituto de Salud Carlos III, Majadahonda, Madrid, Functional Proteomics Unit, Chronic Disease Programme (UFIEC), Instituto de Salud Carlos III, Majadahonda, Madrid, Department of Biochemistry and Molecular Biology, Faculty of Optics and Optometry, Universidad Complutense de Madrid, Madrid



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