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3D Culture Modelling: An Emerging Approach for Translational Cancer Research in Sarcomas

Author(s):

Victoria Heredia-Soto, Andrés Redondo, José Juan Pozo Kreilinger, Virginia Martínez-Marín, Alberto Berjón and Marta Mendiola*   Pages 1 - 11 ( 11 )

Abstract:


Sarcomas are tumours of mesenchymal origin, which can arise in bone or soft tissues. They are rare but frequently quite aggressive and with a poor outcome. New approaches are needed to characterise these tumours and their resistance mechanisms to current therapies, responsible for tumour recurrence and treatment failure. This review is focused on the potential of three-dimensional (3D) in vitro models, including multicellular tumour spheroids (MCTS) and organoids, and the latest data about their utility for the study of important properties for tumour development. We also discuss the use of spheroids as a particularly valuable alternative for compound high throughput screening (HTS) in different areas of cancer biology, which enable the identification of new therapeutic opportunities in commonly resistant tumours.

Keywords:

3D culture, in vitro models, sarcoma, multicellular tumour spheroids, high throughput screening (HTS)

Affiliation:

Translational Oncology Group, IdiPAZ,La Paz University Hospital, Madrid, Translational Oncology Group, IdiPAZ,La Paz University Hospital, Madrid, Molecular Pathology and Therapeutic Targets Group, IdiPAZ,La Paz University Hospital, Madrid, Translational Oncology Group, IdiPAZ,La Paz University Hospital, Madrid, Molecular Pathology and Therapeutic Targets Group, IdiPAZ,La Paz University Hospital, Madrid, Molecular Pathology and Therapeutic Targets Group, IdiPAZ Molecular Pathology Diagnostics Unit, INGEMM La Paz University Hospital, Pº Castellana 261, 28046 Madrid



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