H. Ikeda, J. Kamimoto, T. Yamamoto, A. Hata, Y. Otsubo, T. Niidome, M. Fukushima, T. Mori and Y. Katayama Pages 4419 - 4425 ( 7 )
Phosphatidylcholine-polymer-coated plastic slides were utilized for the fabrication of peptide microarrays for cellular kinome analysis. According to the non-fouling features of the surface, the signal-to-noise ratio of the detection of phosphorylated peptides improved by about 100-fold from that of a peptide microarray fabricated on a glass slide blocked by a commercial BSA-based reagent. When the phosphatidylcholine-polymer-coated peptide microarray was applied to the analysis of the kinome of HCC827 cells, hyperactivation of c-Src and EGFR were successfully detected.
Kinome peptide, microarray, non-fouling, phosphatidylcholine, protein kinase.
, , , , , , , , Department of Applied Chemistry, Kyushu University, 744 Moto-oka Nishi-ku, Fukuoka 819-0395, Japan.